Mutations taking place in earlier generations will increase to larger frequencies by the top of an experiment, when compared with mutations occurring in later generations. thus, the amount of resistant IDENTIFY CRUCIAL GENE MUTATIONS mutants at the end of an experiment will rely not only on the volume of mutations which have transpired, but also on

Treatment with sodium bisulfite deaminates unmethylated cytosine to uracil, Whilst 5mC is not affected. Subsequent PCR amplification will substitute the uracils by thymines. Reads from NGS libraries established in parallel with and without bisulfite procedure are mapped onto reference genomes along with the contrasting C/T positions in between the